Fig 1. In vivo effect of antisense ORNs complementary to S1, S9 and S10 on virus replication.

Schematic representation of S1 (A), S9 (B) and S10 (C) indicating the 5’ and 3’UTRs and the protein coding region (ORF) with the initiation codon (AUG). Positions targeted by the antisense ORNs and 3’UTR length are indicated in each case. (D). Representative examples of plaque assay stained with crystal violet (E). Histogram of virus yield in the presence of different ORNs. S1, S9 and S10 or Scr ORNs were transfected to BSR monolayer cells for 3 hours followed by infection with BTV-1 at 0.1 MOI. At 24hpi virus yield was determined by plaque forming units (PFU) as described in Material and Methods. Values (%) represent the mean and standard deviation of the mean (n = 3–5) generated relative to the control (without ORNs) set at 100%.