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. 2015 Dec 8;11(12):e1005321. doi: 10.1371/journal.ppat.1005321

Fig 3. RNA-RNA interactions between small BTV segments.

Fig 3

(A) Independently transcribed and purified RNA segments (lanes 1 to 4) were heated for two minutes prior incubation in pairs (lanes 5 to 10) as indicated. The mobility shift was analysed by native agarose gel. Interactions are indicated on the right. (B) Simultaneous co-transcription of multiple segments (lanes 5 to 15) as indicated. The positions of the retarded complex and free RNA are indicated on the right (upper panel). Purified and co-transcription reactions, RNA-RNA complexes were quantified by densitometry and expressed as percentage of the ratio of bound and unbound RNA (lower panels of A and B). Values (%) are from the mean and the standard deviation of >3 independent assays (n = 3–5). Individually transcribed segments are shown (lanes 1 to 4, upper panels A and B) as controls. (C) For specificity, multiple co-transcription in the presence of different amounts of yeast tRNA are shown. The position of the bound (retarded RNA complexes) and unbound free RNAs are indicated.