Table 1. The 2’O-methyl modified antisense oligoribonucleotides (ORNs) used for in vivo and in vitro studies.
| ORN | Antisense sequence 5’– 3’ (2’O-methyl modified) | Binding region | Lengthnt |
|---|---|---|---|
| Segment 1 (3944 nt) | |||
| S1 AUG | ACCAUUGCAUUUUAAC | S1 5’ UTR + start codon nt16-1 | 16 |
| S1 3’ | GUAAGUGUAAUGCGGCGCGUGCUC | S1 3’ UTR nt3944-3921 | 24 |
| Segment 9 (1049 nt) | |||
| S9AUG | UGACAUAUGCGAUUUUUUAAC | S9 5’ UTR + start codon nt21-1 | 21 |
| S9.1 | GUAAGUGUAAAAUCGCCCUACGUCAAGAAGGUA | S9 terminal 3’ UTR nt1049-1017 | 33 |
| S9.2 | UUAGAGGUGAUCGAUCAAAUGCAGGAACUCCGUUUUCACA | S9 coding region (3’ term + stop codon) nt1005-966 | 40 |
| S9.3 | CUUCUGUUAGAACUACCCAUCUUCCUCCAUUCGCUCC | S9 coding region (5’ term) nt427-391 | 37 |
| Segment 10 (822nt) | |||
| S10AUG | AUCAGCCCGGAUAGCAUGGCAGCGACACUUUUUAAC | S10 5’ UTR + start codon nt36-1 | 36 |
| S10.1 | GUAAGUGUGUAGCGCCGCAUACCCTCCCCCGUUAGACAGCA | S10 terminal 3’ UTR nt 822–782 | 41 |
| S10.2 | CCUCGGGGCGCCACUCUACCUACUGAUCUUAGGUUAAUG | S10 stop codon to 3’ UTR nt 737–699 | 39 |
| S10.3 | UUAGGUUAAUGGUAAUUCGAAACCAUCUAGCGGGA | S10 coding region (3’term + stop codon) nt709-675 | 35 |
| S10.4 | AAUUUGCUGGUUCAAGCUUCUCUCGCUUUUUGCGC | S10 coding region (3’ term) nt595-561 | 35 |
| S10.5 | GTAGGAGTCTGCATCGTGAGATCAACCACTCTAC | S10 3’UTR nt748-781 | 34 |
| Scrambled (SCR) | UGCUAUUACCAUGCUACAGAUGUAAGUGAU | scrambled sequence | 30 |
The ORN name, sequence (5’-3’), length, target BTV RNA segments regions are listed.