Table 3. Interactions of multiple BTV segments in the presence or absence of ORNs (left panel) and S7, S8, S9 with S10 WT or S10 with deletion mutants (right panel).
| RNA-RNA interactions of segments + ORNs (% of relative RNA retardation) | RNA-RNA interactions of segments with S10 WT or S10 deletion mutants (% of relative RNA retardation) | ||||||
|---|---|---|---|---|---|---|---|
| BTV segments | No ORN | + S10.2 ORN | + S10.5 ORN | BTV segments | WT S10 | ΔS10.2 | ΔS10.5 |
| S7+S8+S9 | 100 | 92 +/- 12 | 96 +/- 8 | S7+S8+S9 | N/A | N/A | N/A |
| S7+S8+S10 | 100 | 33 +/- 10 | 46 +/- 9 | S7+S8+S10 | 100 | 34 +/- 8 | 47 +/- 5 |
| S7+S9+S10 | 100 | 45 +/- 8 | 31 +/- 5 | S7+S9+S10 | 100 | 37 +/- 9 | 47 +/- 5 |
| S8+S9+S10 | 100 | 68 +/- 3 | 89 +/- 15 | S8+S9+S10 | 100 | 105 +/- 9 | 96 +/- 6 |
| S7+S8+S9+S10 | 100 | 42 +/- 7 | 40 +/- 9 | S7+S8+S9+S10 | 100 | 44 +/- 7 | 50 +/- 6 |
Values (%) are the mean and standard deviation of >3 independent experiments relative to control (no ORN or S10 WT) set at 100% (n = 3–5).