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. 2015 Dec 8;10(12):e0143849. doi: 10.1371/journal.pone.0143849

Table 1. Antibodies for immunohistochemical analysis.

Marker Target Vendor (Catalog #) Dilution
Aquaporin 1 (AQP1) Proximal tubule Santa Cruz Biotechnology (sc20810) 1:500
Aquaporin 2 (AQP2) Ascending Loop of Henle, Collecting duct Santa Cruz Biotechnology (sc9882) 1:100
CD31 Endothelial Abcam (ab28364) 1:100
Calbindin (CALB) Ascending Loop of Henle, Distal tubule, Collecting duct EMD Millipore (PC253LEMD) 1:100
Wide-spectrum cytokeratin (CK) Pan-epithelial Abcam (ab9377) 1:100
E-cadherin (ECAD) Collecting duct Dako (M361229) 1:100
Epithelial membrane antigen (EMA) Proximal tubule Dako (M0613) 1:100
Paired box protein 2 (PAX2) Intermediate mesoderm Life Technologies (180483) 1:100
Homeobox protein SIX2 (SIX2) Metanephric mesenchyme Proteintech (11562-1-AP) 1:100
Smooth muscle actin (SMA) Mesangial cells, Vascular smooth muscle EMD Millipore (CBL171) 1:100
Synaptopodin (SYN) Podocyte Progen Biotechnik (61094) 1:100
Uromodulin (UMOD) Loop of Henle Sigma-Aldrich (1400296) 1:100
Vimentin (VIM) Mesenchymal, Interstitial cells Sigma-Aldrich (V6389) 1:100
Wilm’s tumor-1 (WT1) Metanephric mesenchyme, Podocyte Life Technologies (MA5-12481) 1:100