Aquaporin 1 (AQP1) |
Proximal tubule |
Santa Cruz Biotechnology (sc20810) |
1:500 |
Aquaporin 2 (AQP2) |
Ascending Loop of Henle, Collecting duct |
Santa Cruz Biotechnology (sc9882) |
1:100 |
CD31 |
Endothelial |
Abcam (ab28364) |
1:100 |
Calbindin (CALB) |
Ascending Loop of Henle, Distal tubule, Collecting duct |
EMD Millipore (PC253LEMD) |
1:100 |
Wide-spectrum cytokeratin (CK) |
Pan-epithelial |
Abcam (ab9377) |
1:100 |
E-cadherin (ECAD) |
Collecting duct |
Dako (M361229) |
1:100 |
Epithelial membrane antigen (EMA) |
Proximal tubule |
Dako (M0613) |
1:100 |
Paired box protein 2 (PAX2) |
Intermediate mesoderm |
Life Technologies (180483) |
1:100 |
Homeobox protein SIX2 (SIX2) |
Metanephric mesenchyme |
Proteintech (11562-1-AP) |
1:100 |
Smooth muscle actin (SMA) |
Mesangial cells, Vascular smooth muscle |
EMD Millipore (CBL171) |
1:100 |
Synaptopodin (SYN) |
Podocyte |
Progen Biotechnik (61094) |
1:100 |
Uromodulin (UMOD) |
Loop of Henle |
Sigma-Aldrich (1400296) |
1:100 |
Vimentin (VIM) |
Mesenchymal, Interstitial cells |
Sigma-Aldrich (V6389) |
1:100 |
Wilm’s tumor-1 (WT1) |
Metanephric mesenchyme, Podocyte |
Life Technologies (MA5-12481) |
1:100 |