Fig. 1.

Effect of CFTR gene knockout on ouabain-enhanced cyst growth in embryonic kidneys. Embryonic kidneys (E15.5) from Pkd1^m1Bei+/−; Cftr^+/+ and Pkd1^m1Bei+/−; Cftr^−/− mice were isolated and placed into culture in media containing 100 μM 8-Br-cAMP in the absence and presence of 30 nM ouabain. a Representative images of metanephric kidneys in culture. b Average fractional cyst area was calculated after 4 days of treatment. Bars represent the mean ± SEM of 5–8 kidneys depending on the treatment. *P < 0.05 and ^#P < 0.01 versus 8-Br-cAMP treatment alone in the Cftr^+/+ kidneys