Fig. 2.

Effect of CFTR inhibition on ouabain-enhanced ADPKD microcyst growth. Microcyst cultures were established by seeding ADPKD cells within a three-dimensional collagen matrix. Cultures were treated without and with 5 μM forskolin in the absence and presence of 3 nM ouabain. Additionally, some cultures were treated with 10 μM CFTR(inh)-172 and forskolin (5 μM) in the absence and presence of ouabain. Untreated, control cultures remained in defined media alone during the treatment period. After 5–7 days, total cyst surface area of the microcyst cultures was measured. a Representative images of microcyst cultures for each of the indicated treatments. b Average total surface area of microcyst cultures was measured for each treatment condition. Bars represent the mean ± SEM for microcyst cultures grown in sextuplicate with cells from different ADPKD kidneys. *P < 0.05 versus forskolin alone; ^#P < 0.01 versus forskolin + ouabain treatment