Fig. 3.

Effect of CFTR inhibition on net fluid secretion in ADPKD monolayers. Confluent monolayers of ADPKD cells were treated without and with 5 μM forskolin in the absence and presence of 3 nM ouabain. Monolayers were also treated with 10 μM CFTR(inh)-172 and 5 μM forskolin in the absence and presence 3 nM ouabain. Untreated, control monolayers were incubated in media containing no experimental agents. After 24 h, the volume of fluid on the apical side of each monolayer was removed and measured. Data are expressed as fluid secretion rate, μl/h/cm². Bars represent the fluid secretion rate mean ± SEM for monolayers grown in triplicate from different ADPKD kidneys. *P < 0.05 versus forskolin alone; ^#P < 0.01 versus forskolin + ouabain treatment