Fig. 6.

Ouabain effects on the expression of the CFTR regulators PDZK1 and NHERF1 in ADPKD cells. Cells were treated without and with ouabain (3 nM) for 24 h. Following treatment, cells were lysed and equal amounts of cell lysate were separated by SDS-PAGE and then immunoblotted using antibodies specific for human PDZK1 and NHERF1. Protein expression was determined by densitometry. Bars represent protein expression relative to untreated control levels and normalized to tubulin. Data are expressed as the mean ± SEM of three determinations using cells from different ADPKD kidneys. A representative immunoblot is displayed below the graph. *P < 0.05 versus untreated control