(a) Emission spectra of Mito-MPVQ (10 μM) in the presence of various ions in the methanol-water solutions (1:1, v/v, 50mM PBS buffer, pH=7.4). (b) Fluorescence ratio (I488 nm/I420 nm) of Mito-MPVQ upon addition of various metal ions. Experimental conditions: 10 μM Mito-MPVQ, 1 mM Na+, K+ and Ca2+, 10 μM Mg2+, Cr3+, Ni2+, Co2+, Cu+, Cu2+, Fe2+, Mn2+, Cd2+, Zn2+, for λex = 330 nm.