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ACC expression in aortae of C57BL/6 and ApoE−/− mice treated with AICAR. (A) Blots shown are representative. (B) Phosphorylated and (C) total ACC were divided by GAPDH to adjust for protein loading to measure the amount of each form of the enzyme. (D) A ratio of phosphorylated to total ACC was calculated to measure the phosphorylation status of the enzyme. *p < 0.05, **p < 0.01; n = 4.
