Table 3.

Expression of AMPK and ACC in homogenized liver samples from C57BL/6 and ApoE^−/− mice after 14 days of AICAR administration.

	C57BL/6 mice		ApoE −/− mice
Vehicle	AICAR	Vehicle	AICAR
Phospho AMPK	0.54 ± 0.05	0.46 ± 0.03	0.70 ± 0.07	0.58 ± 0.09
Total AMPK	1.01 ± 0.09	0.94 ± 0.14	0.42 ± 0.02⁎⁎	0.46 ± 0.02‡‡
Phosphorylated/total AMPK	0.53 ± 0.02	0.51 ± 0.07	1.68 ± 0.18⁎⁎⁎	1.28 ± 0.05†, ‡‡‡
Phospho ACC	2.07 ± 0.18	1.84 ± 0.27	1.31 ± 0.07⁎	1.24 ± 0.23
Total ACC	1.58 ± 0.18	1.79 ± 0.27	0.98 ± 0.06	1.03 ± 0.15‡
Phosphorylated/total ACC	1.34 ± 0.13	1.03 ± 0.08	1.34 ± 0.07	1.20 ± 0.06

Values for total ACC and phosphorylated ACC are given as a ratio of the density of the band corresponding to the protein of interest relative to the density of the GADPH band. A ratio of phosphorylated to total ACC was used to assess activation of the enzyme. n = 4.

^⁎p < 0.05.

^⁎⁎p < 0.01.

^⁎⁎⁎p < 0.001 vs. C57BL/6 vehicle.

^†p < 0.05 vs. ApoE^−/− vehicle.

^‡‡p < 0.01.

^‡‡‡p < 0.001 vs. C57BL/6 AICAR.