Fig. S1.

Cytochrome P450-4e family expression profiles in Drosophila melanogaster. (A) X-axis shows mean affymetrix microarray signal for each tissue along the y-axis. FlyAtlas data identified three Cyp4e genes, all three show abundant expression in adult Malpighian tubule. (B) D. melanogaster male tubule Cyp4e mRNA expression. Expression levels of Cyp4e mRNA were normalized against the housekeeping reference rp49 and values expressed as fold change relative ± SEM (n = 3). (C) D. melanogaster male and female Cyp4e3 mRNA expression. Female tubule Cyp4e3 mRNA levels were 7-fold higher compared to males. Bars indicated with an asterisk were significantly different (*P = 0.0016 as determined by Student's t-test).