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. 2015 Jun 11;6(26):22152–22166. doi: 10.18632/oncotarget.4158

Figure 5. Detection of phosho-Thr172-AMPK in mitosis.

Figure 5

A. mitotic cell extracts were analysed by immunoblotting. Cells treated by C5M or under control conditions were recovered 4 and 5 hours post release from nocodazole. AMPK and phospho AMPK were detected as well as actin (loading control). B. immunofluorescence were realized on HeLa cells treated by C5M (100 nM, ON) and compared to control conditions (Co). A metaphase, an anaphase and telophases are shown. Survivin is in far-red, actin in red, phospho-Thr172-AMPK in green and DNA in blue. Enlarged views of phospho-AMPK are proposed on the right part. C. detection of phospho-Thr172-AMPK on HeLa or TSA/pc cells stressed by high salt treatment for 10 min at 37°C. Cells pre-incubated with C5M (W C5M 200 nM) for 1 hour before stress induction ([43]) were compared to untreated stressed cells (WO C5M). The signal was turned to a rainbow scale: blue for negative, green to yellow for positive signals and red for the highest signals. Mitotic cells are in red whatever the treatment.