Figure 1. Tivantinib reduces cell viability and colony formation of HCC cells.

A. Effect of increasing concentrations of tivantinib on cell viability as judged by Sybr green assays in the indicated cell lines. B. Colony forming assay showing number of colonies and representative figures of the indicated cell lines. Results are expressed as mean and standard deviation of 3 independent experiments each conducted in triplicate. *p < 0.01; #p < 0.05 vs. control treated cells. C. Western blot analysis of c-MET and p-c-MET in the indicated cell lines. D. Effect of tivantinib on phosphorylation status of c-MET in Huh7 and HepG2 cells after administration of the c-MET ligand HGF. For this experiment, cells were incubated with tivantinib for 24 hours; HGF was added at the concentration of 100 ng/ml for 10 minutes before the cells were harvested.