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. 2015 Jun 10;6(26):22167–22178. doi: 10.18632/oncotarget.4240

Figure 3. Mcl-1 and Bcl-xl play a functional role in determining the apoptotic effect of tivantinib.

Figure 3

A. Effect of Caspase 8 inhibition on tivantinib-mediated apoptosis. FACS analysis was performed to assess nuclear fragmentation 72 hours after administration of tivantinib in the presence or absence of the Caspase8 inhibitor Z-IETD-FMK. B. Effect of Mcl-1 and Bcl-xl silencing by co-transfection of specific siRNA oligonucleotide sequences in Huh7 cells on expression of Bcl-xl and Mcl-1 (each at the concentration of 50 nM); Medium or non-coding siRNA (Ctrl-siRNA) were used as control. C and D. FACS analysis of apoptosis (C), and cell viability (D) after transfection of siRNA targeting Mcl-1 and Bcl-xl. *p < 0.01, in comparison to cells transfected with non-coding siRNA.