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. 2015 Jun 10;6(26):22167–22178. doi: 10.18632/oncotarget.4240

Figure 4. Tivantinib causes a Cyclin B1-dependent G2 cell cycle arrest.

Figure 4

A. Cell cycle quantification and typical flow cytometry pattern of G2/M cell cycle arrest of the indicated HCC cell lines after incubation with tivantinib for 24 hours and PI staining. B. Time kinetic of Cyclin B1 activation. C. Effect of Cyclin B1 silencing by specific siRNA (Cyclin B1-siRNA) on Huh7 cells. Non-coding siRNA (Ctrl-siRNA) was used as control. D and E. Effect of Cyclin B1 silencing on cell cycle (D) and cell viability (E) of Huh7 cells. After overnight incubation, cells were transfected with Cyclin B1-targeting siRNA for 24 h, and then treated with tivantinib for 1 hour before harvesting. Cellular viability was assessed by MTS assay in Huh7 cells after 48 hours. #p < 0.05 vs. cell incubated in medium only or non-coding siRNA.