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. 2015 Jun 2;6(26):22191–22205. doi: 10.18632/oncotarget.4339

Figure 7. GBM cells develop morphological features of endothelial cells when cultured under endothelial-promoting conditions in vitro.

Figure 7

A. Distinct endothelial-like tubular structures comprised of KNS42 cells under normoxia (21% oxygen). B. Better structurally-defined tubules when hypoxia (1% hypoxia) is induced using DFO, but not significantly different in mean number (41.8 +/− 3.6) (p < 0.05) relative to untreated normoxic cultures (45.4 +/− 6.1). C. Moderate impairment of tubular structures upon VEGF inhibition, with significant reduction in mean tubule number (23.4 +/− 4.1 tubules) (p < 0.05) relative to untreated cultures. (D-E) Pronounced impairment of closed tubular structures and reduction of tubule number upon FGFR inhibition (9.5 +/− 3.3) (p < 0.05) and dual VEGF / FGFR inhibition (4.2 +/− 3.3) (p < 0.01). F. Exposure to DMSO alone (FGFR inhibitor vehicle) does not impair tubule formation with no significant difference in mean tubule number (47.6 +/− 8.4) (p < 0.05) relative to untreated normoxia cultures. Representative images were taken 48 hours post-seeding and post-exposure to inhibitors. Scale bar = 50μm.