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. 2015 Jun 10;6(26):22214–22226. doi: 10.18632/oncotarget.4257

Figure 7. IL8 is essential for EGF-induced cell invasion, for MMP9 expression, and for prolonging downstream signaling.

Figure 7

SKBR3 cells were serum-starved for 24 hr and then stimulated with IL8 (10 ng/ml) A. or EGF (50 ng/ml) D. in the absence or presence of the CXCR2 inhibitor SB225002 (2 μM) for the indicated time periods. Total cell lysates were prepared and analyzed for the activation of different signaling pathways by WB using the indicated antibodies. Reproducible results were obtained in three independent experiments. B. Invasion of EGF (50 ng/ml)-stimulated SKBR3 cells through matrigel was assessed by the Boyden chamber assay in the absence or presence of SB225002 (2 μM). Representative images of the invasive cells are shown along with plotted graph demonstrating the mean values ± s.d. from three independent experiments. C. The activity and mRNA level of MMP9 in EGF (50 ng/ml)-stimulated SKBR3 cells in the absence or presence of SB225002 (2 μM) was examined by gelatin zymography and by real-time PCR assays, respectively. The activity and mRNA level were assessed in the conditioned cell medium after 48 hr of incubation in serum-free medium.