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. 2015 Jun 1;6(26):22239–22257. doi: 10.18632/oncotarget.4161

Figure 10. OPN isoform stable cells express different levels of WNT signaling- and EMT-related proteins.

Figure 10

A. Western blot analysis revealed increased E-cadherin expression in OPNa-, OPNb-, OPNib- and OPNic-expressing cells but decreased expression in OPNc-and OPNia-expressing cells as compared to LacZ controls. Vimentin expression was increased in both OPNb- and OPNib-expressing cells but decreased in OPNa-, OPNia-, OPNc-, and OPNic-expressing cells. B. Elevated β-catenin expression was observed in OPNa-, OPNb- and OPNc-expressing cells. Addition of cilengitide did not alter β-catenin expression in any of the OPN isoform-expressing cells but induced E-cadherin expression in OPNc-expressing cells. GAPDH or β-actin was included as a loading control.