Skip to main content
. 2015 Jun 1;6(26):22239–22257. doi: 10.18632/oncotarget.4161

Figure 5. OPNb- and OPNc-expressing stable cells differ in cancer cell invasion.

Figure 5

A. OPNb cells showed significantly more invasion than OPNc cells using Matrigel Basement Membrane Matrix-casted culture dishes (250 μg/ml of BD Matrigel Matrix) following 24–36 h incubation and Diff-Quick staining as compared with cells in non-casted culture dishes. OPN isoform expression levels were monitored using qRT-PCR. B. Matrigel Matrix-resuspended OPNb cells displayed more invasive growth and xenograft formation in vivo than OPNc cells. One million Lenti-Luc-labeled OE33/OPN stable cells were resuspended in 0.1 ml Matrigel Matrix and subcutaneously injected into the flanks of nude mice. In vivo tumor imaging to monitor growth was performed using a Xenogen IVIS Spectrum scanner (*Note, red asterisk, actual imaging intensity should be greater than the reported measurement (total flux, p/s) due to tumor ulceration; blue asterisk, nodule at the site of subcutaneous injection but luciferin signal not detected).