Figure 6. OPN isoform-expressing stable cells differ in cell migration and their response to RGD integrin inhibition.

Flo/OPN stable cells were seeded at 0.6 × 10⁶ cells/12-well, wounded and cultured in media in the presence or absence of cilengitide (100 nM) up to 96 h post wounding. A. In the absence of cilengitide, both OPNb and OPNib cells showed significantly increased migration as compared to LacZ control cells. Both OPNc and OPNic cells were significantly less motile, with gaps remaining more than 96 h post wounding. OPNa cells showed increased migration as compared to LacZ control cells whereas OPNia cells demonstrated similar migration to LacZ cells. B. In the presence of cilengitide, OPNb cell motility was attenuated while OPNib cell migration was severely hindered. Reduced migration of OPNc and OPNic cells remained unchanged. Increased motility of OPNa cells was also attenuated but OPNia cell migration was similar to LacZ cells (*P < 0.05, ***P < 0.001, ****P < 0.0001, t-test).