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. 2015 Jun 1;6(26):22282–22297. doi: 10.18632/oncotarget.4162

Figure 2. Haliclona mucosa extract inhibits resistance of yeast expressing Patched to doxorubicin.

Figure 2

a. Picture of the sponge Haliclona mucosa. b. Yeast expressing Patched were grown in 96 well plates in the presence of 10 μg/mL of the methanolic fraction of Haliclona mucosa crude extract dissolved in DMSO and in the presence or in the absence of 10 μM of dxr. DMSO at a dilution of 1/1000 corresponding to the amount of DMSO added with the sponge extracts was used in the control wells. The growth of yeast was measured by absorbance at 600 nm. The results shown are the mean +/− SEM of three independent experiments and were analyzed using the Student t-test in which significance is attained at P < 0.05 (*) (**: P < 0.005, ***: P < 0.0005) in comparison with the growth of yeast in the presence of dxr and DMSO. c. Compounds purified from Haliclona mucosa methanolic fraction were dissolved in DMSO at 10 mg/mL and added at a final concentration of 10 μg/mL to the growth medium containing or not 10 μM of dxr. DMSO at a dilution of 1/1000 corresponding to the amount of DMSO added with the purified sponge fractions was used in the control wells. The growth of yeast was measured by absorbance at 600 nm. The results shown are the mean +/− SEM of three independent experiments.