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. 2015 Jun 15;6(26):22375–22396. doi: 10.18632/oncotarget.4296

Figure 2. Exonuclease activities of wt and mutant TREX2 variants.

Figure 2

A. SDS-PAGE analysis of the purified TREX2 variants. The migration positions of the molecular weight (MW) standards are indicated. The size difference observed in the R156L variant is due to the plasmid construct used for its cloning and expression, which left six fewer residues at its N-terminus compared to the constructs used for the other variants. B. ssDNA exonuclease activities. C. dsDNA exonuclease activities. Exonuclease titration reactions and time course reactions are shown. The positions of migration of Form II nicked dsDNA (Nicked dsDNA) and circular ssDNA (ssDNA) are indicated. D. Relative ssDNA and dsDNA exonuclease activities.