Figure 6. IMMU-132 mediated pro-apoptosis signaling in human breast cancer lines.

MDA-MB-468 or SK-BR-3 cells were exposed to 1 μM SN-38, the SN-38-equivalent of IMMU-132, or protein equivalent of hRS7 for the indicated times. Cells were harvested and Western blots performed as described in Materials and Methods. Untreated control cells (control) were maintained in growth medium alone until harvested after 48 h. Blots shown are representative of one of two separate experiments.