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. 2015 Jun 5;6(26):22513–22525. doi: 10.18632/oncotarget.4154

Figure 1. Upregulation of lncRNA H19 was observed in mesenchymal-like colon cancer cells.

Figure 1

A. Phase-contrast photomicrographs of the parental and Methotrexate resistant HT-29 cells. B. Relative mRNA levels of E-cadherin and Vimentin were monitored in the parental and Methotrexate resistant HT-29 cells. C. Protein levels of E-cadherin and Vimentin were evaluated in the parental and resistant HT-29 cells. D. The anchorage-independent growth capacity was determined by soft agar assay. E. The cell migration capacity was evaluated by transwell assay. F. The expression levels of candidate lncRNAs were monitored by qRT-PCR. G. HT-29 and SW620 cells were seeded into a 12 well plate and subsequently treated with TGF-β1 for 72 hours. Phase-contrast photomicrographs were captured. H. Protein expression levels of E-cadherin and Vimentin were monitored in HT-29 and SW620 cells to evaluate the effect of TGF-β1. I. qRT-PCR was performed to measure the expression level of H19 after TGF-β1 treatment. *P < 0.05; **P < 0.01; ***P < 0.001.