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. 2015 Jun 5;6(26):22513–22525. doi: 10.18632/oncotarget.4154

Figure 4. H19 functions as a sponge for Vimentin-targeting miRNA.

Figure 4

A. MiR-138 was transfected into HT-29 cells and the mRNA level of Vimentin was evaluated by qRT-PCR. B. After transfection with miR-138, the protein level of Vimentin was examined by western blot. C. Luciferase reporter assay was conducted to test the interaction between miR-138 and Vimentin mRNA using the luciferase reporter containing the coding region of Vimentin. D. The effect of H19 overexpression in the regulation of Vimentin mRNA level was monitored by qRT-PCR. E. The effect of H19 upregulation in the modulation of Vimentin protein level was monitored by western blot. F. Luciferase reporter assay was performed to examine the effect of H19 in antagonizing miR-138-mediated suppression on the coding region of Vimentin. **P < 0.01; ***P < 0.001.