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. 2015 Jun 5;6(26):22513–22525. doi: 10.18632/oncotarget.4154

Figure 5. H19 functions as a decoy for ZEB1- and ZEB2-targeting miRNA.

Figure 5

A. MiR-200a was introduced into HT-29 cells. qRT-PCR was performed to assess the mRNA levels of ZEB1 and ZEB2. B. Western blot was conducted to evaluate the effect of miR-200a in regulating the protein levels of ZEB1 and ZEB2. C. Luciferase reporter assays were utilized to examine the interaction between miR-200a and ZEBs mRNA using reporters containing the 3′UTR region of ZEB1 or ZEB2. D. RT-PCR was used to monitor the effect of overexpression of H19 in the regulation of the mRNA levels of ZEB1 and ZEB2. E. Western blot was utilized to test the effect of overexpression of H19 in modulating the protein levels of ZEB1 and ZEB2. F. Luciferase reporter assay was performed to examine the effect of H19 in attenuating miR-200a-mediated suppression on the 3′UTR region of ZEB1 or ZEB2. **P < 0.01; ***P < 0.001.