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. 2015 Jun 8;6(26):22613–22623. doi: 10.18632/oncotarget.4209

Figure 4. Increased IL-6 levels in tongue epithelium of infected mice.

Figure 4

A. Mice non-infected (white bar) or infected (black bar) with a mixture of P. gingivalis and F. nucleatum every other day for 6 days (as described in Methods) were sacrificed four hours after the last infection. The tongue mucosa was isolated and processed for RNA. Quantitative RT-PCR analysis revealed a 3 fold increase in IL-6 mRNA levels in the tongue mucosa of infected mice. Data are the mean ± SE, *p = 0.014 (Student's t-test). B. Immuno-histochemical analysis with anti-IL-6 antibody revealed expression of IL-6 protein in epithelial compartment of the tongue tissues harvested non-infected and infected mice treated with 4NQO as described in Methods and in Figure 1. Scale bars: 100 μm.