Figure 4. Expression of the p53 protein and its negative regulator MDM2 after simultaneous and sequential combination therapy in the p53 wild type cell line A549.
A. p53 protein levels after treatment B. MDM2 protein levels after treatment; β-actin was used as an internal standard. C. MDM2 mRNA levels after sequential treatment. D. MDM2 mRNA levels after simultaneous treatment. (*p < 0.05: significant difference compared to 0 μM CDDP; **p < 0.05: significant difference compared to 2 μM CDDP).