Table 2. Primer sequences used in this study.
| Gene | Primer sequence (5′-3′) | Anneal .temp. (°C) | No. of cycles | Product size (bp) |
|---|---|---|---|---|
| RT-PCR: | ||||
| GAPDH F | AGAAGGCTGGGGCTCATTTG | 55 | 25 | 287 |
| GAPDH R | AGGGGCCATCCACAGTCTTC | |||
| ASC/TMS1 F | TGG GCC TGC AGG AGATG | 50 | 36 | 411 |
| ASC/TMS1 R | ATT TGG TGG GAT TGC CAG | |||
| MSP: | ||||
| ASC/TMS1 m1 | GCGGGGAGTTTAGGTTTCGTTTC | 62 | 40 | 130 |
| ASC/TMS1 m2 | CCAACGCATCCAAAATAACGTCG | |||
| ASC/TMS1 u1 | GAAGGTGGGGAGTTTAGGTTTTGTTTT | 62 | 40 | 140 |
| ASC/TMS1 u2 | AAATTCTCCAACACATCCAAAATAACAT | |||
| BGS: | ||||
| ASC/TMS1 BGS F | TTG GTG TAA GTT TAGAGATAAGT | 58 | 30 | 557 |
| ASC/TMS1 BGS F | ACC ATCTCCTAC AAACCCATA | |||
| Real Time: | ||||
| ASC/TMS1 F | TCC AGC AGC CAC TCA ACG | 55 | 45 | 66 |
| ASC/TMS1 R | GCA CTT TAT AGA CCA GCA |