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. 2015 May 28;6(26):22767–22775. doi: 10.18632/oncotarget.4082

Figure 1. FBXO22 interacts with KLF4 in vivo.

Figure 1

A. HepG2Cells were transfected with adenovirus expressing Flag-KLF4 or empty vector for 36 hr. The pull-down products from Flag M2 beads affinity purification were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), visualized by Colloidal blue staining, and analyzed by mass spectrometry. The identified SCFFBXO22 components are listed. B–C. Endogenous association between FBXO22 and KLF4 in HepG2 cells was performed by co-immunoprecipitation (CO-IP) experiments. D. The interaction of FBXO22 and KLF4 in cells depleted with KLF4. HepG2 cells were administered with adenoviral shRNA targeting KLF4 or a negative control (NC)