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. 2015 May 28;6(26):22767–22775. doi: 10.18632/oncotarget.4082

Figure 2. FBXO2 promotes KLF4 turnover in HCC cells.

Figure 2

A. The levels of KLF4, KLF2 and KLF5 in cell lysates were determined by Western blot. HepG2 cells were transfected with adenovirus expressing GFP or FBXO22 for 36 hr. B. Ubiquitination of KLF4 in whole-cell lysates was determined by Western blot. C. HepG2 cells were transfected with adenovirus expressing GFP or FBXO22. 36 hr after transfection, the cells were either lysed directly or incubated in the presence of cyclohexamide (50 μg/ml) for the indicated time period in order to determine KLF4 turnover. D. The levels of KLF4 in cell lysates were determined by Western blot. HepG2 cells were transfected with adenoviral shRNA targeting FBXO22 or a negative control (NC) for 36 hr. E. KLF4 turnover was determined by Western blot in HepG2 cells transfected with adenoviral shRNA targeting FBXO22 or a negative control (NC) for 36 hr.