Figure 8. Increased sensitivity of hypoxia-exposed PCA cells towards celecoxib following reoxygenation.

A. LNCaP and B. 22Rv1 cells were exposed to hypoxia for 48 hrs and returned to normoxia with or without celecoxib for 6 days. Clones were counted as detailed in the methods and presented as percentage of control. C. LNCaP cells were exposed to normoxic or hypoxic conditions for 48 hrs; thereafter cells were collected and analyzed for invasiveness in the presence or absence of celecoxib (25 μM). After 22 hrs, number of invasive cells was determined for each group as detailed in the methods. D. EV^Normoxic and EV^Hypoxic were collected from LNCaP cells as detailed in methods. Thereafter, naïve LNCaP cells (100,000 cells per well) were incubated along with 25 μg of EV^Normoxic or EV^Hypoxic in the presence or absence of celecoxib (25 μM) and invasiveness was measured after 22 hrs.*, p ≤ 0.001; #, p ≤ 0.01; $, p ≤ 0.05.