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. 2015 Jun 23;6(25):20801–20812. doi: 10.18632/oncotarget.4600

Figure 4. Cdk2 inhibitor SNS-032, but not Cdk1 inhibitor JNJ-7706621 selectively inhibits growth of ovarian cancer cells with elevated CCNE1 expression by inducing apoptosis.

Figure 4

A. Overnight-cultured cells were harvested and cell lysates were subjected to immunoblotting to detect Cdk1 and Cdk2 protein using the respective antibodies. Membrane was stripped and reprobled with GAPDH polyclonal antibody for loading normalization. B, C. Ovarian cancer cells (40,000 cells/well) were plated in 24-well plates for overnight and varying concentrations of JNJ-7706621 B. or SNS-032 C. were added to cells for 4 days. Cell growth was evaluated by MTT assay and IC50 was estimated as the concentration required for reach 50% growth reduction comparing to untreated cells. D, E. OCC1 D. or OVCAR429 cells E. were treated with 0.5 or 2μM SNS-032 for 2 days, then stained with Annexin V-FITC/propidium iodide and analyzed by FACS. Data are means ± SE. n = 4. *, P < 0.005 vs 0 μM of SNS-032.