Skip to main content
. 2015 Aug 13;6(25):20813–20828. doi: 10.18632/oncotarget.5175

Figure 1. Rab1A is overexpressed in HCC due to copy number increase.

Figure 1

A. Correlation plot of the Rab1A copy number and mRNA level in 187 HCC samples in the TCGA cancer genome database. B. Rab1A mRNA expression in a panel of immortalized liver and HCC cell lines as determined by RT-qPCR. C. Rab1A protein expression in the same panel of immortalized liver and HCC cell lines as determined by immunoblot analysis. GAPDH served as a loading control. D. Correlation plot of Rab1A protein and mRNA expression in the above panel of cell lines. E. MSP results for HCC (MHCC97H and PLC/PRF/5) and immortalized liver (LO2 and QSG-7701) cell lines. M, methylated products of MSP; U, unmethylated products of MSP. F. Upper panel shows the CpG island used for designing primers to detect methylation status of Rab1A locus. Lower panel shows the atlas of PCR fragments for BGS. Arrows indicate potential methylated sites in the LO2 and MHCC97H cell lines. TSS, transcription start site.