Skip to main content
. 2015 Aug 13;6(25):20813–20828. doi: 10.18632/oncotarget.5175

Figure 2. Rab1A overexpression promotes oncogenic growth of HCC in vitro and in vivo.

Figure 2

A. Ectopic Rab1A expression in SK-HEP-1 and BEL7402 cells. Shown is immunoblot analysis of Rab1A expression in ectopic Rab1A-expressing or control vector cells. GAPDH served as a loading control. B. Rab1A overexpression promotes colony formation in HCC cells. SK-HEP-1 and BEL7402 cells overexpressing Rab1A or carrying a control vector were assayed for their ability to form colonies. Results are expressed as mean ± SD of three independent experiments. C. Rab1A overexpression promotes the growth of HCC cells. SK-HEP-1 and BEL7402 cells overexpressing Rab1A or carrying a control vector were analyzed for growth using the CCK-8 assay. Results are expressed as the mean ± SD of three independent experiments. D. Rab1A overexpression promotes HCC tumor growth in xenograft nude mice. Upper panels show images of xenograft tumors at the end of study in nude mice that received a subcutaneous injection of SK-HEP-1 and BEL7402 cells overexpressing Rab1A or carrying a control vector. Lower panels show weights of individual tumors in the two groups. E. H&E and IHC staining for Rab1A in Rab1A-overexpressing and control tumors derived by SK-HEP-1 and BEL-7402 (magnification 200 ×).