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. 2015 Jan 2;6(25):20933–20945. doi: 10.18632/oncotarget.3055

Fig. 1. TRAIP mRNA expression is induced by E2F1 but repressed by Rb.

Fig. 1

Relative mRNA levels of TRAIP (A and C) and CCNE1 (B and D) were measured in HeLa cells 24 (black bars) and 48 (white bars) hours after transfection with the indicated plasmids. In the experiments C and D cells were transfected with 400ng pCMV6E2F1 and 600ng pCMV6RB1 plasmid. Control denotes cells transfected with an empty expression vector. RPL13A was used for normalization in quantitative PCR analysis. Results (mean±SD) were derived from 4 independent experiments. Statistical significance of differences between sample and control values was calculated by two-sided paired t-tests, * p<0.05.