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. 2015 Jan 2;6(25):20933–20945. doi: 10.18632/oncotarget.3055

Fig. 6. TRAIP protein has a short half-life.

Fig. 6

(A) Immunoblot with anti-GFP antibody (upper panel) and GFP-specific qRT-PCR (lower panel) analysis in HeLa cells infected at identical MOI with lentivirus expressing GFP or TRAIP-GFP at 11 days post-transduction. Actin served as loading control in immunoblot and the GFP mRNA level was normalized with RPL13A. (B) Decay of TRAIP-FLAG protein after addition of cycloheximide (50ug/ml) at time 0. TRAIP quantity was normalized with actin. One representative example from several similar experiments is shown.