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. 2015 May 20;6(25):20977–20992. doi: 10.18632/oncotarget.4007

Figure 6. TWIST-1 promotes cell growth and drug resistance depending on c-MPL.

Figure 6

A–C. Cell proliferation (A), colony formation (B) and drug resistance (C) of U937 and K562 cells transfected with c-MPL or empty vector were determined by cell counting, colony-forming assay and cell viability assay, respectively. D–E. Control or shTWIST-1 transfected U937 and K562 cells were further transduced with retrovirus vectors expressing c-MPL-BFP or vector-BFP. Colony formation (D) and cell proliferation (E) were determined by colony-forming assay and cell counting, respectively. F. Western blot analysis of phospho-ERK, phospho-PI3K, phospho-STAT3, phospho-STAT5, ERK, PI3K, STAT3 and STAT5 in the TWIST-1 and c-MPL-transduced K562 and U937 cells. Phosphorylation protein was examined by fold change over total protein and normalized to β-Actin. Asterisks denote significance (**, P < 0.01; ***, P < 0.001).