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. 2015 May 21;6(25):21074–21084. doi: 10.18632/oncotarget.4020

Figure 4. Inhibition of WEE1 induces mitotic catastrophe and inhibits cell growth.

Figure 4

A. WEE1i promotes mitotic catastrophe in HONE1 cells. HONE1 cells were incubated with either buffer or increasing concentrations of WEE1i (100 nM, 250 nM, 500 nM, and 1 μM) for 24 h. Lysates were prepared and the expression of the indicated proteins was detected with immunoblotting. Equal loading of lysates was confirmed by immunoblotting for actin. B. WEE1i promotes mitotic catastrophe in HNE1 cells. HNE1 cells were incubated with either buffer or increasing concentrations of WEE1i (100 nM, 250 nM, 500 nM, and 1 μM) for 24 h. Lysates were prepared and the expression of the indicated proteins was detected with immunoblotting. Equal loading of lysates was confirmed by immunoblotting for actin. C. WEE1i inhibits tumor growth in mouse xenografts. HONE1 cells were injected subcutaneously into nude mice. WEE1i (closed arrow head) was delivered at the indicated time points as described in Materials and Methods. The volume of the tumor was measured on different days (mean ± SD; n = 3).