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. 2015 May 22;6(25):21159–21172. doi: 10.18632/oncotarget.4230

Figure 2. Acadesine phosphorylates VASP and inhibits CXCL12-induced migration.

Figure 2

A. MCL lines (JEKO-1 and HBL-2) and one MCL primary sample were treated with acadesine 2 mM for 6 hours. Phosphorylated and total levels of VASP were analyzed by western blot using α-tubulin as loading control. The ratio between the phosphorylated and unphosphorylated form was showed. B. Actin polymerization of 6 MCL primary samples exposed to acadesine (2 mM) was quantified by flow cytometry at the indicated times after CXCL12 stimulation (200 ng/ml). Values were referred to the corresponding unstimulated sample. Mean ± SEM is represented. *P < 0.05. C. Chemotaxis toward CXCL12 was performed in 6 MCL primary samples after a 3-hour incubation with acadesine 2 mM as detailed in “Methods”. Bars represent the chemotaxis of the viable cells referred to the control cells without CXCL12. Mean ± SEM is represented. *P < 0.05.