Figure 4. Bcl-2 expression inversely correlates with acadesine sensitivity.
A. MCL cell lines (JEKO-1 and HBL-2) and MCL primary samples were treated with acadesine 2 mM for 6 hours. Protein levels of Bcl-2 and Bcl-XL were detected by western blot using α-tubulin as a loading control. B. Correlation between Bcl-2 protein levels assessed by western blot and acadesine cytotoxicity in MCL cell lines (n = 9). Relative Bcl-2 levels were quantified by densitometry using α-tubulin as a loading control. Cytotoxic effect was determined after a 24-hour incubation with acadesine 2 mM and referred to the corresponding untreated control.