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. 2015 Jun 3;6(25):21240–21254. doi: 10.18632/oncotarget.4067

Figure 7. Effect of nutlin-3 and mono-unsaturated lipids on PIP2 / PIP3 levels and on cell death induction.

Figure 7

A. OA and PI36:2 restore PIP3 levels in nutlin-3-treated cells. HCT116 cells were treated with 5 μM nutlin-3 in the absence or presence of 100 μM OA or 10 μM PI36:2. After 72 h, PIP3 levels were measured by a PI(3, 4, 5)P3 Mass ELISA kit (n = 3). Levels are shown as fold change relative to the control group. *p < 0.05 vs nutlin-3, Kruskal-Wallis test. B. PI3 Kinase activity. HCT116 cells were treated with 5 μM nutlin-3 in the absence or presence of 100 μM OA or 10 μM PI36:2. After 72 h, PI3 Kinase activity was determined by measuring the PIP3 product with an ELISA kit (n = 3). C. PIP2 levels were measured using a PI(4, 5)P2 Mass ELISA kit (n = 3). Levels are shown as fold change relative to the control group. ***p < 0.001 vs nutlin-3, Kruskal-Wallis test. D. Cell death was measured using a caspase-3 activity kit. Caspase-3 activity is expressed as the fold change relative to the untreated control group (n = 3). *p < 0.05 by ANOVA. C: control, Nut: nutlin-3, Nut+OA: nutlin + Oleic acid (18:1), Nut+PI: nutlin + PI (36:2).