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Annexin-V/PI double staining was performed on three osteosarcoma cells after treated with ZD6474 at 0, 5, 10 or 20 μM for 48 hours. The graphs are representative of two duplicate experiments, and the percentages of Annexin-V-positive (lower-right quadrant) and Annexin-V/PI double-positive (upper-right quadrant) from these experiments are shown in the relevant quadrants.
