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. 2015 May 24;6(25):21379–21394. doi: 10.18632/oncotarget.4091

Figure 2. In vitro permeability and cellular uptake of GS25 and GS25NP.

Figure 2

A. The permeation of 1 and 5 μg/mL of free GS25 and GS25NP from apical to basolateral across Caco-2 cell monolayers at 37°C, presented as the amount of GS25 transported. B, C. The cellular uptake of GS25 and GS25NP in prostate cancer cells. Cells were treated with B 10 μg/mL of GS25 or GS25NP for 2 h or C 1, 5 or 10 μg/mL of GS25 or GS25NP for 1 h. GS25 was extracted and quantified by a LC-MS/MS analysis and normalized to the protein content. D. Cells were incubated with free coumarin-6 or coumarin-6-loaded NPs for 2 h, then the cellular uptake was monitored by a fluorescence microscope (scale bar, 20 μm). The concentration unit for GS25NP is GS25 equivalent in all experiments and all experiments were repeated three times. (*P < 0.05 and #P < 0.01).