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. 2015 May 24;6(25):21379–21394. doi: 10.18632/oncotarget.4091

Figure 3. In vitro cytotoxicity of GS25 and GS25NP.

Figure 3

LNCaP, DU145, and PC3 cells were exposed to various concentrations of GS25 and GS25NP for 24, 48, or 72 h for determination of A. the cell viability (24 h) and B. IC50 values. The same cell lines were exposed to various concentrations of GS25 and GS25NP for C. 48 h for the determination of cell apoptosis, which was evaluated by the Annexin V-FITC method and D. for 24 h to determine the expression levels of various proteins by Western blotting. The intensity ratio under each band was obtained by IMAGEJ software analysis normalized on untreated control. The concentration unit for GS25NP is GS25 equivalent in all experiments and all experiments were repeated three times. (*P < 0.05 and #P < 0.01).