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. 2015 May 27;6(25):21468–21478. doi: 10.18632/oncotarget.4097

Figure 2. Kapβ2 is required for efficient nuclear translocation of HMW FGF2.

Figure 2

A and B. T98G cells were transfected with scramble siRNA and siRNA against Kapβ2 mRNA. Kapβ2 mRNA and protein levels were examined with RT-PCR (A) and western blot (B) respectively. Data are shown as mean ± SEM of three independent experiments; *P < 0.05 versus control. C. T98G cells expressing HMW FGF2 (HA-HMW) were transfected with scramble siRNA or siRNA against Kapβ2, and subjected to immunostaining by HA antibody (green). Nuclei were stained by DAPI (blue). Scale bar 10 μm. D. T98G cells expressing HMW FGF2 were transfected with scramble siRNA or siRNA against Kapβ2, and subjected to fractionation. Nuclear (N) and cytosolic (C) fractions were analyzed by western blot using antibodies against HA to examine the subcellular distribution of HMW FGF2 protein. GAPDH and Histone H3 were used as marker for cytosolic (C) and nuclear (N) fractions respectively. E. Quantifications of the fluorescence signal of HMW-FGF2 in panel (C) Data are shown as mean ± SEM of three independent experiments; *P < 0.05 versus nuclear; n.s. not significant vs nuclear.