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. 2015 May 27;6(25):21645–21654. doi: 10.18632/oncotarget.4064

Figure 3. miR-27a-3p mimic significantly increased the cell growth, motility and invasion ability in 786-O cells.

Figure 3

A. The proliferation of 786-O cells transfected with the miR-27a-3p mimic or negative control miRNA mimic for 24, 48, 72, 96 and 120 h were examined by MTS assay. Values are means ± S.D. of 6 independent experiments. **p < 0.01 vs. control mimic. B. 786-O cells were transfected with the miR-27a-3p mimic or negative control miRNA mimic for 72 h. Cell migration was measured 7 h after a wound was formed by scraping. Representative results of cell mobility in the scratch wound-healing assay are shown. The results are expressed as means ± S.D. of 6 independent experiments. *p < 0.05 vs. control mimic. C. 786-O cells were transfected with the miR-27a-3p mimic or negative control miRNA mimic for 72 h. The transfected cell suspension was added to the upper chamber of matrigel-coated transwell membrane inserts, and the lower chamber was filled with the complete medium. After 18 h incubation at 37°C, cells that had penetrated the matrigel were fixed with acetone and stained with H&E. Values are means ± S.D. of 6 independent experiments. **p < 0.01 vs. control mimic.