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. 2015 Mar 12;6(25):21740–21754. doi: 10.18632/oncotarget.3226

Figure 6. DU145 Tax-Res exosomes promote DU145 cell invasion.

Figure 6

(A) Quantification of PKH67-labeled, DU145 Tax-Res exosome uptake by DU145 cells after 3 hours and 24 hours of incubation. For control, DU145 cells were incubated with PBS for 24 hours (ctrl). The fluorescence intensity was measured by flow cytometry and the percentage of positive cells was measured by the manufacturer's software (means ± SD, n = 3, ***P < 0.05); (B) Extracellular matrix degradation (ECM) assay on DU145 Tax-Sen cells cultured in the presence of either PBS, 10 μg/ml of DU145 Tax-Res or 10 μg/ml DU145 Tax-Sen cell derived exosomes for 24 hours. DU145 Tax-Res exosomes (10 μg/ml) were cultured together with the ECM-fluorescein for 24 hours. The regions of interest were identified and quantified as described in materials and methods. The DU145 Tax-Sen cells were co-stained with phalloidin for actin cytoskeleton and with DAPI for the nucleus, (C) The ECM degradation was quantified as described in materials and methods, (means ± SD, n = 3, ***P < 0.05); (D) Immunoblot analysis of total and phosphorylated levels of AKT and ERK1/2 in DU145 Tax-Sen cells cultured in the presence or absence of 10 μg/ml of DU145 Tax-Res cell derived exosomes for 24 hours.